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Kumar SV, Tarun P, Kumar TA. Journal of Physical Chemistry, 58(11), 953-957. (2008). Advances in chemistry series Washington, DC: American Chemical Society., pp. De Belder, A.N. Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. Advances in Carbohydrate Analysis, 1, 63-144. In addition, the permeation rate was higher in pig skin than in human skin. The numerical value of a indicates that dextrans acquire the shape of a rigid sphere in aqueous solution; and kM-H demonstrates that under water the value decreases with temperature [71, 72]. MeSH terms Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Hennink, W.E. WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. 10K, and c M.W. Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. Carbohydrate Polymers, 79, 380-390. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. 2011;16(2):518. Please enable it to take advantage of the complete set of features! (2008). 2018;1:10915. Table 2 compares the properties of nanoparticles coated with one or other polymer. Formation of dextran hydrogels by crystallization. ; Aberle, T; Burchard, W. (2000). Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. Journal of Controlled Release, 119, 5-24. Durand, A. ; Patel, T.R. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. Seven dextrans, provided by Sigma, with molecular weight of 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa. Named after George Gabriel Stokes, it is closely related to solute mobility, factoring in not only size but also solvent effects.A smaller ion with stronger hydration, for example, may have a greater Stokes radius than a larger ion with HHS Vulnerability Disclosure, Help Macromolecules, 29, 2265-2268. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Gekko, K. (1971). Citation-(RIS Figure3 shows a magnified image of human cadaver skin with microchannels formed by the microporation device (250kHz) and the skin closure over time. The characteristic Rg/Rh value for a globular protein is ~0.775, which means that Rg is smaller than Rh. 43-1, 115-147. Pharmaceut Sci Tech. NCI CPTC Antibody Characterization Program. Where f0 is the frictional coefficient of a sphere having the same hydrodynamic (hydrated or solvated) volume V as the particle. Alternatives to animal testing: a review. Amersham Biosciences, AA Edition, www.amershambiosciences.com. The hydrodynamic radius is important in predicting transretinal penetration Carroll PJ. Polysaccharide hydrogels for modified release formulations. The Mark-Houwink. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. ; Garca de , J. This result could reflect the presence of some aggregated species within the lysozyme sample solution. Springer Nature. ; Ghosh, T.K. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). Dextrans can function efficiently as anterograde or retrograde tracers, Boudry I, Blanck O, Cruz C, Blanck M, Vallet V, Bazire A, Capt A, Josse D, Lallement G. Percutaneous penetration and absorption of parathion using human and pig skin models in vitro and human skin grafted onto nude mouse skin model in vivo. Journal Non-Newtonian Fluid Mech., 97, 295-301. Dextran Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. The receptor phase was collected immediately (time 0) and 2, 4, 8, 12, and 24h. This receptor phase solution was transferred to a 96-well plate and the skin-permeated FITC-dextran was measured using a microplate reader (Molecular Devices, Co. Ltd., Sunnyvale, CA, USA) at 405nm. ; Dutt, N.V.K. Takara, Andres; Acosta, Adolfo; Masuelli, Martin A. government site. Microporation applications for enhancing drug delivery. The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. IV. National Library of Medicine Enhanced topical delivery of small hydrophilic or lipophilic active agents and epidermal growth factor by fractional radiofrequency microporation. Gillespie, T.; Hulme, M. A. The micropores of the human cadaver skin were regenerated according to the time. and transmitted securely. Synthesis of water-soluble and water-insoluble amphiphilic derivatives of dextran in organic medium. Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. Trop J Pharm Res. Modelling the endothelial blood-CNS barriers: a method for the (2012). Drug Deliv Syst. Carbohydrate Polymers, 36, 319-326. The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24). ; Prasad, D.H.L. In a poor solvent which shows a positive heat of mixing, segments of a polymer molecule attract each other in solution more strongly than attract the surrounding solvent molecules. dextran hydrodynamic American Journal of Food Science and Technology 2013, 1(3), 60-66. We observe that this maximum radius is below Nanoparticles in magnetic resonance imaging: from simple to dual WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. 2015 Mar 17;6:155. doi: 10.3389/fmicb.2015.00155. Strategies to improve the potential of transdermal devices by enhancing the skin permeation of therapeutic entities. Dilute solution properties and degree of chain branching The human skins were from the back or thigh of a 24-year-old woman. 2023 BioMed Central Ltd unless otherwise stated. sharing sensitive information, make sure youre on a federal A solvent at this temperature is called a theta solvent [53]. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. Determination of constants, kM-H and a, from the intrinsic viscosity data, requires a series of monodisperse polymer samples with known molecular weight or a series of polydisperse polymer samples with known viscosity average molecular weights, Mv. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. statement and The fact that the intrinsic viscosity of a given polymer sample is different according to the solvent used gives and insight about the general shape of polymer molecules in solution. Molecular weight determination of polysaccharides. Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. At 25C both values of a and kM-H are within the range reported by [3, 8, 16]. "Dextrans in Aqueous Solution. 2011;5(2):95132. Polymers | Free Full-Text | Confinement-Induced Fractionation and Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. WebDiffusivity of 2000 kDa Dextran was significantly lower for both hydrogel formulations, but was insensitive to collagen concentration, likely due to a larger hydrodynamic diameter ( dh ~ 70 nm) on order of the collagen mesh size. (2003). Journal of Applied Polymer Science, 13, 2031-2032. TEWL measurements were performed before and 30min after microporation. Guner, Ali (1999). Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. pp13-21. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. Naik A, Kalia YN, Guy RH. Viscosity of aqueous solutions of polysaccharides and hydrophobically modified polysaccharides: Application of Fedors equation. Masuelli, Martin Alberto. The skin poration was performed using the microporation device (LG electronics, Korea). These results indicate that healing and regeneration occurred in the human cadaver skin after the microporation. A novel correlation for protein diffusion coefficients based on molecular weight on radius of gyration. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. High Manufacturing Standards of Molecular Probes Dextrans. Front Microbiol. Global hydrodynamic analysis of the molecular flexibility of galactomannans. Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. 20K groups (n=3, *: p<0.05, **: p<0.01). Eng. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Universal size-independent quantities for the conformational characterization of rigid and flexible macromolecules. The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. Proc. Viscotek GmbH, Europe. Microneedles and their applications. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. Webprotein hydrodynamic radius was observed with increasing protein concentration (in the range 0.25-2 mM) in both the absence and presence of 8 M urea. Ind. A simple and rapid method for evaluation of MarkHouwinkSaturada constants of linear random coil polysaccharides using molecular weight and intrinsic viscosity determined by high performance size exclusin chromatography: application to guar galactomannan. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. J Cosmet Laser Ther. The regeneration process of the skin is regulated by growth factors such as keratinocyte growth factor and fibroblast growth factor, which trigger the proliferation and migration of cells [23, 24]. It is a measure of the width of molecular weight distributions (MWD) as well as probability of molecular weight distribution curve (GPC). What is hydrodynamic radius The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). (2013). ; Bataille, B. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules.